Design an experiment that would allow for the identification of photosynthetic organisms.
Explain that light energy is captured in chemical bonds through the process of photosynthesis.
Draw the links between photosynthesis and cellular respiration.
Explain the growth requirements of photosynthetic organisms.
Construct a growth curve.
Compare growth curves to draw conclusions.
Vision and Change Core Concepts and Competencies (http://visionandchange.org)
Core Concept:
Pathways and transformations of energy and matter: Biological systems grow and change by processes based upon chemical transformation pathways and are governed by the laws of thermodynamics.
Core Competencies:
Ability to apply process of science: Biology is evidence based and grounded in the formal practices of observation, experimentation, and hypothesis testing.
Ability to use quantitative reasoning: Biology relies on applications of quantitative analysis and mathematical reasoning.
Biocore Guide (Brownell et al., https://doi.org/10.1187/cbe.13-12-0233)
Transformations of Energy and Matter: Energy captured by primary producers is stored as chemical energy. This stored energy can be converted into ATP, which is required for energetically demanding activities necessary for life, including synthesis, transport, and movement.
Next Gen Science Standards (http://www.nextgenscience.org/)
The process of photosynthesis converts light energy to stored chemical energy by converting carbon dioxide plus water into sugars plus released oxygen. (HS-LS1-5)
The sugar molecules thus formed contain carbon, hydrogen, and oxygen: their hydrocarbon backbones are used to make amino acids and other carbon-based molecules that can be assembled into larger molecules (such as proteins or DNA), used for example to form new cells. (HS-LS1-6)
Photosynthesis and cellular respiration (including anaerobic processes) provide most of the energy for life processes. (HS-LS2-3)
The main way that solar energy is captured and stored on Earth is through the complex chemical process known as photosynthesis. (secondary to HS-LS2-5)
ASPB – BSA Core Concepts and Learning Objectives in Plant Biology for Undergraduate: https://aspb.org/wp-content/uploads/2016/05/ASPB-BSA-CoreConcepts.pdf
Plants capture light energy to assimilate inorganic carbon dioxide into organic compounds.
Plants capture and use energy from sunlight. Almost all other organisms on the planet eat plants as a source of energy.
Process of Science Skills, Pelaez, N, et al. “The Basic Competencies of Biological Experimentation: Concept-Skill Statements“ (2017). PIBERG Instructional Innovation Materials. Paper 4. http://docs.lib.purdue.edu/pibergiim/4
Posing problems
Generating hypotheses
Designing experiments
Identification of proper controls
Comparison requires varying only one thing at a time
Testing hypotheses
Unexpected results used to modify hypothesis
Interpreting/evaluating data
Visual representations used to help interpret data
Photosynthesis is only a function of (multicellular) plants.
The purpose of photosynthesis is to produce oxygen.
The purpose of photosynthesis is to “make” energy.
Photosynthetic organisms do not themselves use the sugar/starch they produce (it is only consumed by others).
Photosynthetic organisms do not have mitochondria and do not produce ATP through oxidative respiration.
All microorganisms require “food” to grow.
It is not easy to determine how many cells are in a culture because you cannot see them without a microscope
Green algae contain chloroplasts and perform photosynthesis.
The purpose of photosynthesis is to capture energy (from sunlight) to drive cellular functions.
The sugar that photosynthetic organisms produce can be metabolized when they need energy.
Photosynthetic organisms do not require external sugar for growth, since they make their own from CO2 and H2O.
Photosynthetic organisms require sunlight, water, CO2 and trace elements (small amounts of P, Mg, N, S, Ca, etc.) for growth.
The concentration of green algae in solution is directly related to the intensity of green color of the solution, and reflects increasing cell numbers as the algae grows.
Hartsock, AK. Killing Chloroplasts: Herbicides Targeting Photosynthesis. National Center for Case Study Teaching. http://sciencecases.lib.buffalo.edu/cs/collection/detail.asp?case_id=893&id=893
The electron transport game (Cornely, K. Biochem Ed. 1999. 27: 74-76) may be played in the context of a plant cell. We’ve expanded the game to include an ATP synthase and a chloroplast. Furniture in the classroom is moved around to generate a “membrane barrier”, with ping pong balls used as protons to couple the ATC and ATP synthase.
Acknowledgement
This material is based in part upon work supported by National Science Foundation (NSF) grants 1432286 and 1432303. Any opinions, findings and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
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