Fermentation
  • Environmental conditions (O2) influence metabolic pathways
MINT Learning Objectives
  • Articulate that both fermentative and respiratory pathways begin with glycolysis.

  • Recognize the link between glycolysis and fermentation and describe why if one process were to stop, the other process could not continue either.

  • Recognize that while fermentative pathways may have different products, they always allow cells to regenerate NAD+ which is necessary for glycolysis.

  • Recognize that respiration requires molecular oxygen in order to further oxidize carbon compounds and generate cellular energy.

  • Describe the link between oxygen concentration and metabolic flux in the fermentation and respiratory pathways.

  • Recognize that metabolic processes take time and concentration of products can change over time.

  • Compare ATP production from glycolysis (substrate level phosphorylation) and oxidative phosphorylation with growth rate and overall growth density of organisms.

National Standards Alignments:
  • Vision and Change Core Concepts and Competencies (http://visionandchange.org)

    • Core Concept:

      • Pathways and transformations of energy and matter: Biological systems grow and change by processes based upon chemical transformation pathways and are governed by the laws of thermodynamics.

    • Core Competencies:

      • Ability to apply process of science: Biology is evidence based and grounded in the formal practices of observation, experimentation, and hypothesis testing.

      • Ability to use quantitative reasoning: Biology relies on applications of quantitative analysis and mathematical reasoning.

  • Biocore Guide (Brownell et al., https://doi.org/10.1187/cbe.13-12-0233)

    • Transformations of Energy and Matter: Energy captured by primary producers is stored as chemical energy. This stored energy can be converted through a series of biochemical reactions into ATP for immediate use in the cell.

    • Transformations of Energy and Matter: In cells, the synthesis and breakdown of molecules is highly regulated. Biochemical pathways usually involve multiple reactions catalyzed by enzymes that lower activation energies. Energetically unfavorable reactions are driven by coupling to energetically favorable reactions such as ATP hydrolysis.

    • Evolution: Most organisms have anatomical and physiological traits that tend to increase their fitness for a particular environment.

    • Information Flow: Organisms have sophisticated mechanisms for sensing changes in the internal or external environment. They use chemical, electrical, or other forms of signaling to coordinate responses at the cellular, tissue, organ, and/or system level.

  • American Society for Microbiology Curriculum Guidelines (https://www.asm.org/)

    • The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.

    • The growth of microorganisms can be controlled by physical, chemical, mechanical, or biological means.

    • The regulation of gene expression is influenced by external and internal molecular cues and/or signals

    • Microorganisms and their environment interact with and modify each other.

  • Process of Science Skills, Pelaez, N, et al. “The Basic Competencies of Biological Experimentation: Concept-Skill Statements“ (2017). PIBERG Instructional Innovation Materials. Paper 4. http://docs.lib.purdue.edu/pibergiim/4

    • Posing problems

      • Includes library research

    • Generating hypotheses

      • New knowledge incorporated with old to form new hypotheses

    • Designing experiments

      • Identification of proper controls

      • Comparison requires varying only one thing at a time

    • Testing hypotheses

      • Unexpected results used to modify hypothesis

    • Interpreting/evaluating data

      • Visual representations used to help interpret data

    • Determining follow-up

Interactive Video Vignette Information
IVV Title: To Ferment or Not to Ferment: That is the Question
IVV URL for Students:  
  • Copy the URL to the Clipboard. Either paste it into an email to your students, or use it to create a link in your course management system.
IVV Description
Two undergraduate biology students are puzzling over the results from a microbiology experiment meant to determine if a bacterial strain is capable of fermentation or not. They reason their way through the problem, set up, and carry out another experiment to test their ideas. In the end, they are able to come to an understanding about the relationship of two key metabolic pathways.
Novice Ideas and IVV Learning Goals
Novice Ideas
  • Fermentation is a separate pathway/can happen in the absence of glycolysis.

  • Pathways are isolated from other pathways (students often miss the concept of a network).

  • Organisms are programmed to do either respiration or fermentation (conditions of the organism’s environment do not influence metabolic pathways).

  • The product of a reaction’s appearance is all or none (not recognizing that products can build up over time and cause a change.)

  • Fail to think of a reaction as having a rate, or one that will change as the reactants are used up.

Ideas addressed in the IVV
  • Fermentation and glycolysis are linked pathways.

  • Overview of glycolytic and fermentation pathways.

  • Presence of oxygen influences which pathway is used – respiration vs. fermentation.

  • Metabolism of cells may alter the pH of the growth medium.

  • Organisms may use the same pathways, but do so at different rates.

  • Growth rate and density are linked to metabolism.

Recommended In-class Curricular Material
Please see the MINT FAQ page for general information on the use of MINTs and IVVs with your class. The following curricular materials are provided as examples of resources that may be used in class to further student learning towards IVV and MINT learning objectives.
  • Bergan-Roller HE, Galt NJ, Dauer JT, Helikar T. 2017. Discovering Cellular Respiration with Computational Modeling and Simulations. CourseSource. https://doi.org/10.24918/cs.2017.10

  • Yung SB, Primm TP. Active Learning for Basic Metabolic Pathways.  J Microbiol Biol Educ. 2014 15(2):319-320. 

Assessment Question Information
The research team has developed multiple select questions for assessing IVV effectiveness. Please contact the research team if you are interested in assessing IVV use in your course.
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Acknowledgement

This material is based in part upon work supported by National Science Foundation (NSF) grants 1432286 and 1432303. Any opinions, findings and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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